In-vitro and in-vivo antioxidant potential and GC-MS profile of Argemone mexicana L.

Abstract

The present study was founded on callus induction, antioxidant activity and thermostable secondary metabolite profiling in the callus and plant parts of an unexplored medicinal plant, Argemone Mexicana L. Callus was induced from young stem of A. mexicana on MS medium supplemented with different concentrations and combinations of PGRs such as NAA (0.5-1.0 mg/L), KN (0.5-2.0 mg/L) and BAP (0.5-1.5mg/L). The best degree of callus induction was recorded on MS medium containing 1.0mg/L NAA+0.5mg/L BAP and 1.0mg/L NAA+2.0mg/L KN. The free radical scavenging assay (antioxidant activity) using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and ferric ion reducing antioxidant power (FRAP) in various fractions of A. mexicana leaves, stem and callus indicated higher content of flavonoids (0.0656±0.0008 mg RUT (Rutin equivalent)/100g-dw) and phenolics (1.63±0.94 mg GAE (Gallic acid equivalent)/100 g-dw conc.) in vitro (callus) compared to different plant parts (explants)advocating strong antioxidant potential of the plant. In vitro too, the methanolic extracts obtained from A. mexicana callus and stem explants through GC-MS profiling showed richness of various important antioxidants such as1,3-propanediol,2-(hydroxymethyl)-2-nitro-; Squalene;6-alpha-cadina-4, 9-diene,9,(-)-;Stigmast-5-en-3-ol(3-beta);Vitamin-E; 1(2-H)-Naphthalenoneoctahydro-4a-8a-dimethyle-7(1-methyle); 1, 2-benzendicarboxylic acid in callus and Benzene propanoic acid; 3,5-bis (1,1- dimethylethyl)-4-hydrox); Neophytadiene; Di-isooctyles; Hexadecane and Dibutyl phthalate, in stem, respectively. These bioactive compounds may be utilized in treating various ailments such as inflammation, rheumatism, cancer, and jaundice, etc.