Efficient regeneration from cotyledonary leaf explants of a medicinal herb Corynandra chelidonii var. pallae (Angiosperms: Cleomaceae)

Abstract

Corynandra chelidonii var. pallae (Angisperms: Eudicots: Malvids) is a rare, endemic, and medicinally useful herb with gradually declining natural populations over the years. It was chosen for in-vitro regeneration in this study. An efficient protocol was developed using cotyledonary leaf explants from 10 to12 day-old in-vitro grown seedlings. The explants were inoculated on a shoot induction medium containing different concentrations with a combination of BAP, IAA, NAA, and coconut water. High efficiency shoot regeneration (21.62 ± 0.35a) was achieved in cotyledonary leaf with MS medium supplemented with 2.0 mg/l BAP 0.2 mg/l NAA + 10% coconut water. The response obtained was highest in shoot elongation on MS medium with 2.0mg/l GA3 and 0.5 mg/l TDZ, with a 5.78 cm average shoot length. The MS medium supplemented with 1.0 mg/l IBA was found the best for root regeneration from in-vitro developed shoots. The regenerated plantlets with the well-developed shoot and root system have acclimatized successfully. The above mentioned protocol could be successfully used for conservation and for large-scale multiplication; and is the first of its kind in this taxon.