Nutrition, age of explant and plant growth regulators affect the multiple shoot induction in Lantana camara L

Abstract

We undertook the present study to investigate the factors affecting multiple shoot induction in Lantana camaraL. Among all the explants used only shoot-tip explants derived from 1- and 2-week-old seedlings could form multiple shoots. BAP(6- Benzylaminopurine) was the most effective PGR (plant growth regulator) in inducing multiple shoots. Similarly, IBA(Indole-3- butyric acid) efficiently induced root in the microshoots. In addition, salts and sugars also affected regeneration efficiency. MS medium supported induction of multiple shoots the most followed by B5, SH and WPM medium in that order. Likewise, among sugars sucrose and glucose were the best for the induction of microshoots. Our findings show that the MS medium containing 3% sucrose and 4.4 μM BAPinduce maximum multiple shoots in 2-week-old shoot-tip explant. The shoots root over MS medium containing either 4.9 μM IBAor 5.5 μM IAA(Indole-3-acetic acid).