Microscopical study and characterization of brachysclereid (stone cell) In Manilkara zapota (L.) P. Royen (Sapotaceae) fruit pulp
Keywords:
Manilkara zapota, G-lignin, S-lignin, FTIR, UV-VIS spectrophotometer, Brachysclereid, Fluorescence.Abstract
Microscopical study and characterization of brachysclereid in Manilkara zapota fruit pulp has been discussed here. Isodiametric shape of brachysclereid is present in sapodilla fruit pulp (Mesocarp). The amount of brachysclereid in fruit pulp is 3.2 gm. Chemical composition of brachysclereid (stone cell) is mainly composed of lignin. Mostly Guaiacyl lignin (G-lignin), and syringyl lignin (S-lignin) is present. This lignin has been identified by Fourier Transform Infrared Spectroscopy (FTIR) and UV-VIS spectroscopy method. The absorption peak at 288 nm. of UV-VIS spectrophotometer confirmed the G-lignin. The FTIR band of 1377cm-1 indicate the C-O stretching of syringyl ring and the band 1262 cm-1 indicate the C-O stretching of Guaiacyl ring. The Wiesner reagent and maule treatment also confirmed the presence of G-lignin. Under UV light the brachysclereid shows fluorescence signal.
