EFFECT OF DIFFERENT SURFACE STERILIZING AGENTS AND GROWTH MEDIA ON IN VITRO SEED GERMINATION OF LIPPIA NODIFLORA L.

Abstract

Lippia nodiflora is a fast-growing, small creeping perennial herb that belongs to the family Verbenaceae. L. nodiflora is used for ethnomedicinal and pharmacological applications like adenopathy, chronic indolent ulcers, knee joint pain, cold, fever, anticancer, antidiabetic, and gastrointestinal disorder. This study was aimed to optimize the in vitro seed germination protocol to address. Seeds were sterilized with different surface sterilizing agents at different time period. Sterilized seeds were inoculated on different strengths of MS basal medium with various levels of sucrose concentrations. By comparison of measured characteristic results, we found that the maximum percentage of seed germination was recorded as 85.26 ± 0.04 on ½ strength MS basal medium supplemented with 1% (w/v) sucrose when the seeds were surface sterilized with 0.1% HgCl for 5 minutes. We optimized the seed surface sterilization 2 protocol and culture condition for the first time that may be useful for regeneration, transformation, and conservation studies.